These days, one of the most common diseases among the world’s population is related to bone injuries. Thus, mimicking natural bones through making polymer/inorganic composite biomaterials would be a good way to produce implants to replace the damaged bone tissues [1, 2]. One of the most useful composite biomaterials are bioactive glasses (BGs) that are special glass systems, generally composed of SiO2, CaO, P2O5, and Na2O [3-6].
BGs may produce compliant and strong bond between the glass and the tissue through complex biochemical and chemical reactions, which makes them good candidates for clinical applications in a variety of situations [3, 7, 8]. Meanwhile, nanosized 58S BG is a popular biomaterial due to its excellent biological properties like stimulating angiogenesis in vitro. Some preparation methods are surfactant assisted sol-gel method , sol-gel , coprecipitation  and powder metallurgy .Between the proposed routes, sol-gel offers a very advantageous method because of producing high purity and homogenous materials at lower processing temperatures. It’s been said that, the BG powders produced through this technique are more bioactive than other routes [12-20]. However, due to strong acidic phosphorous resource, preparing these nanosized 58S BGs with controlled morphology, composition, size and size distribution is still difficult [1, 21, 22].
It’s been demonstrated that Ag2O has antibacterial activity and biological impacts [3, 23-25]. Sharifianjazi et al. , stated that SiO2-P2O5-CaO-Ag2O bioactive glasses with 2 mol% of Ag2O exhibited a higher bioactivity than 4 mol% and 6 mol% of Ag2O, and this sample, after 14 days, apatite morphology was perfectly dispersed glacier shaped, which had not been previously reported by anyone .
In another work accomplished by Sharifianjazi et al. , it’s been reported that the rate of crystalline HA formation on SiO2-P2O5-CaO-SrO-Ag2O bioactive glass containing 5% Ag2O, was the highest in comparison with other specimens . Additionally, it’s been illustrated that the presence of low silver contents (3% and 5% Ag2O mol?) has terrific effect on promoting both differentiation and proliferation of G292 osteoblastic cells .
Furthermore, it’s been said that Zinc has an important role in bone cell growth and DNA replication [28, 29]. Additionally, Zinc stimulates the synthesis of proteins and is cofactor for many enzymes [30-33]. Phetnin et al.  prepared samples belonging to the 80SiO2-(15 – x)CaO-5P2O5-xAg2O system where 0
In this work, we used a sol-gel method to synthesize nanosized 58S BG substituted by Ag and Zn particles, to find out the effect of simultaneous substitution of Ag and Zn on bioactivity and biocompatibility of the produced nanoparticles.